| Microorganism name |
Escherichia coli
|
| Target proteins |
Human unhydroxylated collagen II (Guo et al., 2010)
|
| Wild-type or GMO |
GMO (Guo et al., 2010)
|
| Production mode (intracellular/extracellular) |
Intracellular (Guo et al., 2010)
|
| Protein yield (g/L or g/g?) |
10.8 g/L (Guo et al., 2010)
|
| Temperature used in study |
34°C (Guo et al., 2010)
|
| pH used in study |
pH 6.8 (Guo et al., 2010)
|
| C & N source |
Glucose, yeast extract & (NH4)2SO4 (Guo et al., 2010)
|
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
Collagen production by E.coli K-12 S9188 has FDA GRAS approval in the US
|
| Companies |
|
| Publications/references |
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Guo, J., Luo, Y., Fan, D., Yang, B., Gao, P., Ma, X., & Zhu, C. (2010). Medium optimization based on the metabolic‐flux spectrum of recombinant Escherichia coli for high expression of human‐like collagen II. Biotechnology and Applied Biochemistry, 57(2), 55–62. https://doi.org/10.1042/ba20100081
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Augustin, M. A., Hartley, C. J., Maloney, G., & Tyndall, S. (2023). Innovation in precision fermentation for food ingredients. Critical Reviews in Food Science and Nutrition, 64(18), 6218–6238. https://doi.org/10.1080/10408398.2023.2166014
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Gomes, A. M. V., Carmo, T. S., Carvalho, L. S., Bahia, F. M., & Parachin, N. S. (2018). Comparison of Yeasts as Hosts for Recombinant Protein Production. Microorganisms, 6(2), 38. https://doi.org/10.3390/microorganisms6020038
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Eastham, J. L., & Leman, A. R. (2024). Precision fermentation for food proteins: ingredient innovations, bioprocess considerations, and outlook — a mini-review. Current Opinion in Food Science, 58, 101194. https://doi.org/10.1016/j.cofs.2024.101194
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Bajić, B., Vučurović, D., Vasić, Đ., Jevtić-Mučibabić, R., & Dodić, S. (2022). Biotechnological Production of Sustainable Microbial Proteins from Agro-Industrial Residues and By-Products. Foods, 12(1), 107. https://doi.org/10.3390/foods12010107
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Spohner, S. C., Müller, H., Quitmann, H., & Czermak, P. (2015). Expression of enzymes for the usage in food and feed industry with Pichia pastoris. Journal of Biotechnology, 202, 118–134. https://doi.org/10.1016/j.jbiotec.2015.01.027
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Urniezius, R., Masaitis, D., Levisauskas, D., Survyla, A., Babilius, P., & Godoladze, D. (2023). Adaptive control of the E. coli-specific growth rate in fed-batch cultivation based on oxygen uptake rate. Computational and Structural Biotechnology Journal, 21, 5785–5795. https://doi.org/10.1016/j.csbj.2023.11.033
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Bauer, S., & Shiloach, J. (1974). Maximal exponential growth rate and yield of E. coli obtainable in a bench‐scale fermentor. Biotechnology and Bioengineering, 16(7), 933–941. https://doi.org/10.1002/bit.260160707
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Förster, A. H., & Gescher, J. (2014). Metabolic Engineering of Escherichia coli for Production of Mixed-Acid Fermentation End Products. Frontiers in Bioengineering and Biotechnology, 2. https://doi.org/10.3389/fbioe.2014.00016
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